| Annotated protein: | Dynamin (EC 3.6.5.5) (Protein shibire) (dDyn). Gene symbol: SHI. Taxonomy: Drosophila melanogaster (Fruit fly). Uniprot ID: P27619 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DNM1 SynGO data @ DNM2 SynGO data @ DNM3 |
| Ontology domain: | Biological Process |
| SynGO term: | presynaptic dense core vesicle exocytosis (GO:0099525) |
| Synapse type(s): | Neuro-muscular junction |
| Annotated paper: | Wong MY, et al. "Synaptic neuropeptide release by dynamin-dependent partial release from circulating vesicles" Mol Biol Cell. 2015 Jul 1;26(13):2466-74 PMID:25904335 |
| Figure(s): | Figure 1 |
| Annotation description: | To test whether dynamin affects activity-dependent neuropeptide release at the Drosophila NMJ, GFP-tagged prepro-Drosophila insulin-like peptide 2 (Dilp2) was expressed in wild-type (WT) animals and the temperature-sensitive dynamin mutant shits1. Release at the larval neuromuscular junction was evoked by tetanic nerve stimulation (70 Hz, 1 min) at 22°C (i.e., a permissive temperature) or after heating to 30°C to acutely inactivate shits1 Figure 1 A and B: Temperature change did not affect synaptic neuropeptide release in WT animals. Figure 1 C and D: Temperature-dependent dynamin inhibition halved activity-evoked peptide release. Temperature-dependent dynamin inhibition does not affect presynaptic Ca2+ responses to activity (Macleod et al., 2004). Therefore, at a native synapse, dynamin directly enhances activity-evoked peptide release. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Wide-field fluorescence |
| Annotator(s): | Dnyanada Sahasrabudhe (ORCID:0000-0003-2916-7616) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | Figure 3: Nerve terminals of shi larvae, depleted of synaptic vesicles, exhibit robust Ca2+ signals at nonpermissive temperatures indicating that Shi larvae do not affect the intracellular Ca2+ signalling in inducing DCV exocytosis. @ PMID:15014125 |
| SynGO annotation ID: | 4064 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |