| Annotated protein: | Segment polarity protein dishevelled homolog DVL-1 (Dishevelled-1) (DSH homolog 1). Gene symbol: DVL1. Taxonomy: Mus musculus (Mouse). Uniprot ID: P51141 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DVL1 |
| Ontology domain: | Cellular Component |
| SynGO term: | neuronal dense core vesicle (GO:0098992) |
| Synapse type(s): | spinal cord |
| Annotated paper: | Zhao B, et al. "Transport of receptors, receptor signaling complexes and ion channels via neuropeptide-secretory vesicles" Cell Res. 2011 May;21(5):741-53 PMID:21321602 |
| Figure(s): | Figure 3 and 4 |
| Annotation description: | Dvl1 was detected in Liquid chromatography-mass spectrometry results of LDCVs. Figure 3B: Post-embedding immunogold (inset) staining against Dvl1 displays localisation on the surface of LDCV. Figure 4: Dvl1-myc was transfected in PC12 cells, immunostaining demonstrates Dvl1 localisation on vesicular structures indicating that Dvl1 is a vesicular protein. Figure 3A and S2D: Fzd8 immunoreaction was present on LDCVs and did not colocalise with SP or CGRP (DCV markers) indicating that LDCVs might have another subset that does not have SP or CGRP. 18/01/2021 Dnyanada: The paper does not provide any information on the specificity of the antibody used. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Wide-field fluorescence Mass-spectrometry |
| Annotator(s): | Dnyanada Sahasrabudhe (ORCID:0000-0003-2916-7616) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4046 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |