Annotated protein:Voltage-dependent calcium channel subunit alpha-2/delta-1 (Voltage-gated calcium channel subunit alpha-2/delta-1) [Cleaved into: Voltage-dependent calcium channel subunit alpha-2-1; Voltage-dependent calcium channel subunit delta-1]. Gene symbol: CACNA2D1. Taxonomy: Mus musculus (Mouse). Uniprot ID: O08532
antibody wiki:
SynGO gene info:SynGO data @ CACNA2D1
Ontology domain:Cellular Component
SynGO term:neuronal dense core vesicle (GO:0098992)
Synapse type(s):spinal cord
Annotated paper:Zhao B, et al. "Transport of receptors, receptor signaling complexes and ion channels via neuropeptide-secretory vesicles" Cell Res. 2011 May;21(5):741-53 PMID:21321602
Figure(s):Figure 1, 2
Annotation description:Cacna2d1 was detected in Liquid chromatography-mass spectrometry results of LDCVs.
Figure 1: Subcellular fractionation shows immunoblotting of Cacna2d1 in LDCVs fraction.
Figure 2A: Double immunofluorescence staining shows the distribution of Cacna2d1(green) with Substance P positive LDCV (red).
Figure 2B: Post-embedding immunogold (inset) staining against Cacna2d1 displays localisation on the surface of LDCV.

18/01/2021 Dnyanada: The paper does not provide any information on the specificity of the antibody used.
Evidence tracking, Biological System:Intact tissue
Evidence tracking, Protein Targeting:Antibody (detection)
Evidence tracking, Experiment Assay:Electron Microscopy
Wide-field fluorescence
Mass-spectrometry
Biochemical fractionation (generic)
Annotator(s):Dnyanada Sahasrabudhe (ORCID:0000-0003-2916-7616)
Guus Smit (ORCID:0000-0002-2286-1587)
Matthijs Verhage (ORCID:0000-0002-2514-0216)
Lab:Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
SynGO annotation ID:4042
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology