| Annotated protein: | Synaptosomal-associated protein 25 (SNAP-25) (Super protein) (SUP) (Synaptosomal-associated 25 kDa protein). Gene symbol: SNAP25. Taxonomy: Mus musculus (Mouse). Uniprot ID: P60879 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SNAP25 |
| Ontology domain: | Biological Process |
| SynGO term: | presynaptic dense core vesicle exocytosis (GO:0099525) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Arora S, et al. "SNAP-25 gene family members differentially support secretory vesicle fusion" J Cell Sci. 2017 Jun 1;130(11):1877-1889 PMID:28404788 |
| Figure(s): | Figure 1, 2 and 4 |
| Annotation description: | Figure 1C-F: WT neurons (black bars) showed robust DCV fusion after the first AP burst while fusion in SNAP-25 KO (red bars) neurons was strongly impaired. The blue bars represent stimulation (16×50AP@50Hz). Figure 1H: The DCV fusion probability, defined as the number of fusion events/total DCV number per cell, was strongly reduced in SNAP-25 KO neurons compared to WT. Figure 2 D-K: The significant reduction in DCV number and fusion probability is seen only at DIV4. Figure 4 A, C-D: Lentiviral expression of SNAP-25 in SNAP-25 KO neurons fully rescued DCV fusion capacity with a similar number of fusion events and a fusion probability as that in WT neurons. Figure 4E: The DCV fusion rate (number of fusion events over time) was similar between WT and SNAP-25 KO neurons expressing SNAP-25. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Wide-field fluorescence pHluorin assay |
| Annotator(s): | Dnyanada Sahasrabudhe (ORCID:0000-0003-2916-7616) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4033 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |