| Annotated protein: | Reticulon-4 (Foocen) (Glut4 vesicle 20 kDa protein) (Neurite outgrowth inhibitor) (Nogo protein). Gene symbol: RTN4. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9JK11 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RTN4 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | spinal cord |
| Annotated paper: | Liu YY, et al. "Electron microscopic localization of Nogo-A at the postsynaptic active zone of the rat" Neurosci Lett. 2003 Aug 7;346(3):153-6 PMID:12853107 |
| Figure(s): | Figure 1 |
| Annotation description: | Figure 1: Electron micrographs showing RTN4 immunoreactivity in the spinal cord motoneurons of adult rats, detected with pre-embedding immunogold-silver cytochemistry (A-C) and post-embedding immunogold cytochemistry (D). Immunogold particles indicating RTN4 immunoreactivity could be found in dendritic profiles (Den) and in close apposition to the postsynaptic density (thin arrows in A-C) and the postsynaptic dense bodies (thin arrows in C and D) at both symmetric (thick arrow in A and B) and asymmetric synapses (thick arrow in C and D). Note on the antibody used: Antibody was generated against an RTN4 specific C-terminus 572-amino acid fragment (621-1192). The antibody is further described in PMID: 12577319. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Dnyanada Sahasrabudhe (ORCID:0000-0003-2916-7616) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | This paper characterises specificity of the antibody used against RTN4. Figure 1: Western blot of the lysate of the mock plasmid transfected COS-7L cells showed negative staining with the RTN4 antibody (lane 1 of Fig. 1A). That of the COS-7L cells with full-length hnogo-A (human RTN4) transfection displayed several bands, with the topmost band at 220 kDa, which matched the size of Nogo-A (lane 2 of Fig. 1A). Western blot of the spinal cord showed a very strong band at 220 kDa and a faint band at the lower end (lane 3 of Fig. 1A). Figure 3C: Omission of primary antibody shows no staining in ventral horn of spinal cord. @ PMID:12577319 |
| SynGO annotation ID: | 4021 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |