| Annotated protein: | Reticulon-4 (Neurite outgrowth inhibitor) (Nogo protein). Gene symbol: RTN4. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q99P72 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RTN4 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Annotated paper: | Berry S, et al. "Loss of Nogo-A, encoded by the schizophrenia risk gene Rtn4, reduces mGlu3 expression and causes hyperexcitability in hippocampal CA3 circuits" PLoS One. 2018 Jul 24;13(7):e0200896 PMID:30040841 |
| Figure(s): | Figure 3 |
| Annotation description: | Figure 3 A: Assessment of the paired-pulse ratio of EPSCs recorded in CA3 pyramidal neurons, as an indicator of synaptic release probability, revealed no difference between WT and RTN4 -/- cells. This raises the possibility of an absence of a presynaptic effect of RTN4 KO in the synapse. Figure 3 B: Double labelling experiments showed that RTN4 colocalises with dendritic marker MAP2. Figure 3C: EM studies show RTN4 immunoreaction in the dendrites and spines, but not in the presynaptic compartment. Figure S1: WT and RTN4 -/- mouse slices were stained with the antibody used for RTN4 staining. The absence of staining in the KO conditions indicates its specificity. 12/01/2021 Dnyanada: The EM images used in the study aren't of the highest quality, they simply demonstrate a homogenous distribution of the RTN4 immunoreaction product in the dendrites and spines, and lack of it in the presynapse. Based on this, the ontology term "postsynapse" was used. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal Electrophysiology (generic) |
| Annotator(s): | Dnyanada Sahasrabudhe (ORCID:0000-0003-2916-7616) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4020 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |