| Annotated protein: | Regulating synaptic membrane exocytosis protein 2 (Rab-3-interacting molecule 2) (RIM 2) (Rab-3-interacting protein 2). Gene symbol: RIMS2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9EQZ7 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RIMS2 |
| Ontology domain: | Biological Process |
| SynGO term: | structural constituent of active zone (GO:0098882) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Wang SSH, et al. "Fusion Competent Synaptic Vesicles Persist upon Active Zone Disruption and Loss of Vesicle Docking" Neuron. 2016 Aug 17;91(4):777-791 PMID:27537483 |
| Figure(s): | Figure 1, 2, 3, 4, 5, 7 |
| Annotation description: | It is demonstrated that RIM2 is required to maintain the structural integrity of the active zone. Genetic removal of active zone proteins RIM1, RIM2, ELKS1 and ELKS2 leads to a loss of Bassoon, Munc13 and Piccolo, and a reduction of RIMBP2 and Cav2.1 (Figure 1), thus showing a severe reduction of the active zone. In line with disruption of the active zone, multiple essential functions of the presynaptic terminal are affected, including vesicle docking (figure 2), vesicle priming (figure 7), spontaneous and action potential induced fusion (figure 3 and 5) and calcium influx (figure 4). Removal of RIM1/2 or ELKS1/2 alone is not sufficient to disrupt the active zone (figure 7). 10/4/2017 Pim - I have added the link to SynGO ID #116 that shows that RIM2 is itself localized to the AZ. I think this strengthens the selected term. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal Whole-cell patch clamp Western blot |
| Annotator(s): | Arthur de Jong (ORCID:0000-0002-7620-2704) Pascal Kaeser (ORCID:0000-0002-1558-1958) |
| Lab: | Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA |
| Additional literature: | Acuna et al. shows a similar but milder phenotype in a mouse line where RIM1, RIM2, RIMBP1 and RIMBP2 are removed. @ PMID:27537484 |
| SynGO annotation ID: | 401 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |