| Annotated protein: | Anoctamin-6 (Small-conductance calcium-activated nonselective cation channel) (SCAN channel) (Transmembrane protein 16F). Gene symbol: ANO6. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q6P9J9 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ANO6 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of synaptic membrane (GO:0099699) |
| Synapse type(s): | spinal cord, acetylcholinergic |
| Annotated paper: | Soulard C, et al. "Spinal Motoneuron TMEM16F Acts at C-boutons to Modulate Motor Resistance and Contributes to ALS Pathogenesis" Cell Rep. 2020 Feb 25;30(8):2581-2593.e7 PMID:32101737 |
| Figure(s): | fig 2 |
| Annotation description: | "TMEM16F / Anoctamin-6 could be a post-synaptic component of C-boutons" Several experiments in Fig 2 show that TMEM16F protein is an alfa-motoneuron marker, which faces cholinergic C-boutons synapses. - "By staining TMEM16F was observed in choline acetyltransferase-positive (ChAT+) motoneurons, specifically located in the ventral horn laminae VIII and IX. This protein can be observed forming clusters at the plasma membrane of soma and dendrites (Figs. 2A and B)" - "Co-staining of TMEM16F with a plasma membrane marker of motoneurons (Na+/K+-ATPase alfa1) is shown in (Fig. 2F) verifying its localization. In Fig 2G, TMEM16F protein is observed on the opposite side to the presynaptic marker Synaptophysin, which could indicate a postsynaptic localization, although strong evidence would be necessary. Moreover, TMEM16F clusters are found facing the vesicular acetylcholine transporter (VAChT) pre-synaptic clusters (Fig. 2I), being VAChT a marker of synaptic cholinergic C-boutons. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Carlos Pascual-Caro (ORCID:0000-0002-2345-393X) Anjali Amrapali Vishwanath (ORCID:0000-0002-9888-0928) Jaime de Juan-Sanz (ORCID:0000-0002-1212-5623) |
| Lab: | ICM - Brain and Spine Institute, Hpital Piti Salptrire, 47 Boulevard de l'Hpital, 75013 Paris, France |
| SynGO annotation ID: | 4009 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |