| Annotated protein: | Adenylyl cyclase-associated protein 2 (CAP 2). Gene symbol: CAP2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9CYT6 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CAP2 |
| Ontology domain: | Biological Process |
| SynGO term: | presynaptic actin cytoskeleton organization (GO:0099140) |
| Synapse type(s): | cerebral cortex |
| Annotated paper: | Kumar A, et al. "Neuronal Actin Dynamics, Spine Density and Neuronal Dendritic Complexity Are Regulated by CAP2" Front Cell Neurosci. 2016 Jul 26;10:180 PMID:27507934 |
| Figure(s): | Figure 6, 8 and 9 |
| Annotation description: | Figure 6B: Immunocytochemistry study shows an increase in F-actin accumulation in the soma and neurite processes in CAP2 mutant. Quantification of WT and CAP2 mutant neurons with this aberrant pattern of F-actin distribution indicated defective F-actin distribution in CAP2 mutant phenotype. Figure 6C: Quantification of F-actin fluorescence intensity indicated an increased F-actin accumulation in CAP2 mutant as compared to WT. CAP is known to play a role in dissociation of the cofilin-G-actin-ADP complex for actin polymerisation (Moriyama and Yahara, 2002). To study if CAP2 interacts with cofilin in neurons: Figure 8: Pull-down assays with varying concentrations of GST-CAP2 and purified n-cofilin indicated an interaction between the proteins. Figure 9A: Western Blotting showed that the amount of phosphorylated n-cofilin was lower in CAP2 mutants as compared to WT. Figure 9B: Immunofluorescence analysis also revealed a lower intensity of phosphorylated n-cofilin in CAP2 mutants as compared to WT. Figure 9C: Immunohistochemistry showed that CAP2 mutant had an aberrant pattern of n-cofilin distribution in the form of cytoplasmic aggregates as compared to the normal distribution in the cytoplasm and nucleus in case of WT Taken together, CAP2 mutants demonstrate actin accumulation and impaired actin dynamics (in terms of F-actin and activated n-cofilin distribution) The SynGO term presynaptic cytoskeletal organisation was used based on the co-localisation studies of CAP2 with synapsin I (shows overlap in the presynaptic terminal, Supplementary S1C, D) and PSD-95 (localises adjacent to PSD-95, Figure 1E) The 'regulate' box was ticked because CAP2 affects the amount of phosphorylated n-coffilin (Figure 9A). |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot IP + WB/MSMS |
| Annotator(s): | Dnyanada Sahasrabudhe (ORCID:0000-0003-2916-7616) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4006 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |