| Annotated protein: | Tetraspanin. Gene symbol: TSPAN7. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: B0BNE7 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ TSPAN7 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic neurotransmitter receptor endocytosis (GO:0099149) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Bassani S, et al. "The X-linked intellectual disability protein TSPAN7 regulates excitatory synapse development and AMPAR trafficking" Neuron. 2012 Mar 22;73(6):1143-58 PMID:22445342 |
| Figure(s): | Figure7 A,B and Figure 8A |
| Annotation description: | Figure 7, A and B: Fluorescence microscopy targeted towards intracellular and total GluA2 was used to calculate the GluA2 internalisation index. TSPAN7 Knockdown demonstrated an increase in the GluA2 internalisation index indicating its role as a regulator in GluA2 trafficking in the post-synapse. To ascertain that the effect was a true representation of endocytosis of GluA2 receptor, a similar experiment in the presence of dynamin inhibitor Dynasore was carried out. Dynasore abolished the differences in the internalization index between TSPAN7-knockdown and scrambled siRNA14 neurons. Figure 8 A: Rescue WT rescues the effect of TSPAN7 knockdown by reducing the internalization index close to control levels. Overexpression of TSPAN7 reduces the GluA2 internalization index compared to control. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Wide-field fluorescence |
| Annotator(s): | Dnyanada Sahasrabudhe (ORCID:0000-0003-2916-7616) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4002 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |