| Annotated protein: | Rho-related GTP-binding protein RhoB. Gene symbol: RHOB. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P62747 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RHOB |
| Ontology domain: | Cellular Component |
| SynGO term: | synapse (GO:0045202) |
| Synapse type(s): | cerebral cortex |
| Annotated paper: | Doussau F, et al. "A Rho-related GTPase is involved in Ca(2+)-dependent neurotransmitter exocytosis" J Biol Chem. 2000 Mar 17;275(11):7764-70 PMID:10713089 |
| Figure(s): | Figs 1 and 2 |
| Annotation description: | Immunoblotting of rat brain subcellular fractions indicates the presence of RhoB in synaptosomes. 11/01/2021 Dnyanada: The paper detects RhoB in the crude synaptic vesicle fraction (Sy) and the membrane-bound compartment fraction (Sy Mb) of the rat brain using a polyclonal antibody against RhoB. The quality of the images used in the additional literature field (PMID: 20203211) isn't optimal; and the juxtaposition with synaptophysin & colocalisation with PSD95 isn't robust to qualify the annotation to the SynGO term 'postsynapse'. Based on this, the generic term "synapse" was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Western blot Biochemical fractionation (generic) |
| Annotator(s): | John Chua (ORCID:0000-0002-5615-1014) |
| Lab: | Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117597, Neurobiology/Ageing Program, Life Sciences Institute, National University of Singapore and Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore |
| Additional literature: | Fig 3. Immunocytochemical labelling of cultured corticohippocampal neurons dually labelled for RhoB with synaptophysin or PSD95. RhoB signals show apposition with synaptophysin and colocalisation of RhoB with PSD95, indicating postsynaptic localisation of RhoB. @ PMID:20203211 |
| SynGO annotation ID: | 3994 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |