| Annotated protein: | Ras-related protein Ral-A (EC 3.6.5.2). Gene symbol: RALA. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P63322 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RALA |
| Ontology domain: | Cellular Component |
| SynGO term: | synaptic membrane (GO:0097060) |
| Annotated paper: | Han K, et al. "Regulated RalBP1 binding to RalA and PSD-95 controls AMPA receptor endocytosis and LTD" PLoS Biol. 2009 Sep;7(9):e1000187 PMID:19823667 |
| Figure(s): | Fig S2F and S2G |
| Annotation description: | Subcellular fractionation of rat brain indicates the presence of RalA in synaptosomes and synaptic membrane fractions. 18/01/2021 Dnyanada: The paper demonstrates the presence of RalA in PSD fractions (Fig S2F), however, the term "postsynaptic density" was not chosen due to a decrease in signal intensity observed as the fractions become PSD specific (Fig S2G; contrary to the pattern elicited by PSD95). The additional literature cited lie in favour that RalA is indeed a synaptic protein. Figure S2F shows enrichment of RalA in the synaptic membrane fraction (LP1) and was used as the basis for the ontology term. Figure 9C-D shows specificity of anti-RalA antibody by a reduced signal in RalA-/- brain extracts. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Western blot Biochemical fractionation (generic) |
| Annotator(s): | John Chua (ORCID:0000-0002-5615-1014) |
| Lab: | Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117597, Neurobiology/Ageing Program, Life Sciences Institute, National University of Singapore and Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore |
| Additional literature: | RalA is detected in MS analyses of isolated NMDA receptor complexes. @ PMID:10862698 RalA is detected in MS analyses of isolated PSD fraction. @ PMID:15020595 |
| SynGO annotation ID: | 3989 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |