| Annotated protein: | Neurocan core protein (Chondroitin sulfate proteoglycan 3). Gene symbol: NCAN. Taxonomy: Mus musculus (Mouse). Uniprot ID: P55066 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NCAN |
| Ontology domain: | Cellular Component |
| SynGO term: | perisynaptic extracellular matrix (GO:0098966) |
| Synapse type(s): | neocortex, GABAergic |
| Annotated paper: | Sullivan CS, et al. "Perineuronal Net Protein Neurocan Inhibits NCAM/EphA3 Repellent Signaling in GABAergic Interneurons" Sci Rep. 2018 Apr 18;8(1):6143 PMID:29670169 |
| Figure(s): | Fig. 2G and F |
| Annotation description: | Fig. 2F shows immunogold labeling in the vicinity of an inhibitory axon terminal onto neuronal soma documenting Neurocan as component of the perisynaptic extracellular matrix. Fig2G shows specificity of the neurocan antibody where COS7 cells were transfected to express neurocan fused to alkaline phosphatase (Neurocan-AP), or alkaline phosphatase (AP) alone. Only cells expressing Neurocan-AP were positive for labeling. The AP transfected cells and control lacking primary antibody showed no signal, indicating specificity of the antibody. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Daniela Dieterich (ORCID:0000-0002-9880-1214) Rainer Pielot (ORCID:0000-0002-9681-3318) Karl-Heinz Smalla (ORCID:0000-0002-0269-0311) Eckart Gundelfinger (ORCID:0000-0001-9377-7414) |
| Lab: | Leibniz Institute for Neurobiology (LIN), Institute of Pharmacology and Toxicology, Otto von Guericke University, Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany |
| Additional literature: | NCAN_RAT was also found in other PSD preparations (suppl. Table S1). @ PMID:14720225 |
| SynGO annotation ID: | 3985 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |