| Annotated protein: | Vacuolar protein sorting-associated protein 35 (Maternal-embryonic 3) (Vesicle protein sorting 35). Gene symbol: VPS35. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9EQH3 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ VPS35 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynapse (GO:0098793) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Vazquez-Sanchez S, et al. "VPS35 depletion does not impair presynaptic structure and function" Sci Rep. 2018 Feb 14;8(1):2996 PMID:29445238 |
| Figure(s): | Fig1, S2 |
| Annotation description: | Fig 1A and B show that endogenous VPS35 highly overlaps with VAMP2, indicating that VPS35 is localised to synapses. Fig1C-F show by immuno EM that VPS35 is predominantly localised to presynaptic terminals (quantification in Fig1D), but is not present in all presynaptic terminals (Fig1C) in line with the not-complete overlap observed in confocal studies (Fig 1A-B). Information on Antibody validation: Fig S2a: Negative control processed in parallel with the immunolabelling with VPS35 Cat. No. ab10099, but preincubating the primary antibody with the blocking peptide Cat. No. ab23181 at a ratio 5:1. Fig S2b-c: Negative control processed in parallel with the immuno-gold labelling with VPS35, but without adding primary antibody, just bridging antibody and Protein A-gold. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal |
| Annotator(s): | Jan van Weering (ORCID:0000-0001-5259-4945) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 3980 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |