| Annotated protein: | Adhesion G protein-coupled receptor L3 (Latrophilin-3) (Lectomedin-3). Gene symbol: ADGRL3. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q80TS3 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ADGRL3 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic membrane (GO:0099055) |
| Synapse type(s): | hippocampus, glutamatergic Schaffer collateral synapse (CA3->CA1) |
| Annotated paper: | Sando R, et al. "Latrophilin GPCRs direct synapse specificity by coincident binding of FLRTs and teneurins" Science. 2019 Feb 22;363(6429):eaav7969 PMID:30792275 |
| Figure(s): | Fig 1b, fig. S2a, Fig. S2b, Fig. 3b,d-g |
| Annotation description: | The authors describe the localization of N-terminal genetically tagged HA-tagged Latrophilin-3 from the endogenous locus. HA-Latrophilin-3 is expressed postsynaptically in excitatory neurons, a conclusion based on exemplary images shown: In hippocampal cultured neurons, HA-Latrophilin-3 was present in excitatory (positive for CamkIIa) but not inhibitory synapses (Fig. S2a). In slices of the hippocampus, HA-Latrophilin-3 colocalized with excitatory marker vGlut1 in Stratum radiatum of CA1 region (and not with inhibitory marker vGAT) (Fig. 3d, e) and was present in CA1 neurons of S. oriens (Fig. 3b). When HA-Latrophilin-3 was abolished in sparse neurons in cultures (Fig. 1b; Fig. S2b) or in CA1 region of the hippocampus (Fig. 3f,g), these neurons were absent of HA-Latrophilin-3 staining indicating postsynaptic expression. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Tony Cijsouw (ORCID:0000-0003-0912-1514) Thomas Biederer (ORCID:0000-0002-3670-7863) |
| Lab: | Department of Neuroscience, Tufts University School of Medicine, Boston, MA 02111, USA |
| SynGO annotation ID: | 3977 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |