| Annotated protein: | Ras-related protein Rap-2a (EC 3.6.5.2) (RbBP-30). Gene symbol: RAP2A. Taxonomy: Homo sapiens (Human). Uniprot ID: P10114 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RAP2A |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic membrane neurotransmitter receptor levels (GO:0099072) |
| Synapse type(s): | Schaffer collateral synapse (CA3->CA1) |
| Annotated paper: | Zhu Y, et al. "Rap2-JNK removes synaptic AMPA receptors during depotentiation" Neuron. 2005 Jun 16;46(6):905-16 PMID:15953419 |
| Figure(s): | Fig. 1, 2, 5, 6 |
| Annotation description: | Over-expressed GFP-tagged Rap2 in acute rat hippocampal slices is localized to dendrites and spines (Fig.1). The use of constitute-active (ca) and a dominant-negative (dn) mutants of Rap2 indicate that Rap2 activity enhances synaptic depression (50% depressed AMPA-receptor-mediated responses in the Rap2(ca) mutant (Fig. C2), and potentiated AMPA responses in the Rap2(dn) mutant (Fig. 2 A, B)). Rap2 activity further induces synaptic depotentiation which prevents the induction of LTP (Fig. 5) by mediating signalling that ultimately leads to the removal of synaptic GluR1- and GluR2L-containing AMPA receptors (Fig. 6). 19/01/2021 Dnyanada: Rap2 mutant constructs, including the constitutively active mutant (G12→E), dominant-negative mutant (S17→N), and null/dead mutant (F39→S) were generated as described in Machida et al., 2004. Literal: cDNA fragment encoding amino acids 1-168 of human Rap2A was subcloned into plasmid pBTM116 to yield pBTM116-Rap2A. P10114 (RAP2A_HUMAN) was chosen based on this. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Two-photon microscopy Whole-cell patch clamp |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 3975 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |