| Annotated protein: | Ras-related protein Rap-2a (EC 3.6.5.2) (RbBP-30). Gene symbol: RAP2A. Taxonomy: Homo sapiens (Human). Uniprot ID: P10114 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RAP2A |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of synapse assembly (GO:0051963) |
| Annotated paper: | Chen X, et al. "Rap2 and TNIK control Plexin-dependent tiled synaptic innervation in C. elegans" Elife. 2018 Jul 31;7:e38801 PMID:30063210 |
| Figure(s): | Fig. 1, 2, 4 |
| Annotation description: | C. elegans RAP-2 GTPase (rap-2) is involved in regulating synapse formation. RAP-2 null mutants and gain-of-function mutants (constitutive GTP-form of rap2) exhibit synaptic tiling defects indicating that the cycling between GTP- and GDP-forms is required for the regulation of spatial patterning of synapses (Fig. 1). Expression of rap-2 in the postsynaptic muscle cells cannot rescue the tiling defect, whereas presynaptic expression in DA neurons can rescue the phenotype (Fig. 2). Fluorescence Lifetime Imaging Microscopy (FLIM)-based FRET (Förster Resonance Energy Transfer) measurements using human EGFP-Rap2A were performed to test the localization of Rap-2 activity with the help of a construct (mRFP-RaIGDS(RBD)-mRFP) that specifically binds to GFP-Rap2, but not GDP-Rap2 (Assay explained in Fig. 4A). Human Rap2 had higher activity in synaptic regions compared to non-synaptic domains (Fig. 4). 19/01/2021 Dnyanada: As Human Rap2a cDNA was used for generating RAP2A mutant c.elegans lines, P10114 (RAP2A_HUMAN) was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Over-expression |
| Evidence tracking, Experiment Assay: | Confocal FRET |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 3974 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |