| Annotated protein: | A-kinase anchor protein 5 (AKAP-5) (A-kinase anchor protein 150 kDa) (AKAP 150) (P150) (cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein). Gene symbol: AKAP5. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P24587 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ AKAP5 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density, intracellular component (GO:0099092) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Colledge M, et al. "Targeting of PKA to glutamate receptors through a MAGUK-AKAP complex" Neuron. 2000 Jul;27(1):107-19 PMID:10939335 |
| Figure(s): | Fig1; Fig2c,d; Fig3b; Fig4, Fig5b,d,e; Fig6c,d; |
| Annotation description: | By using pulldown assays from heterologous expression of recombinant proteins and from rat synaptosomal preparations, researchers have shown that AKAP150/79 is localized in the postsynaptic density by direct binding to PSD-95 and SAP97. Moreover, these interactions also target AKAP150/79 to NMDA and AMPA receptors, respectively. Researchers also show that AKAP150 colocalizes with PSD-95 in immunostained primary hippocampal neurons using confocal microscopy. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) IP + WB/MSMS |
| Annotator(s): | Maria Andres-Alonso (ORCID:0000-0002-1585-539X) Michael Kreutz (ORCID:0000-0003-0575-6950) |
| Lab: | RG Neuroplasticity, Leibniz Institute for Neurobiology, 39118 Magdeburg, Germany and Leibniz Group 'Dendritic Organelles and Synaptic Function', Center for Molecular Neurobiology, ZMNH, University MC, Hamburg-Eppendorf, Hamburg, 20251, Germany |
| SynGO annotation ID: | 396 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |