| Annotated protein: | Regulating synaptic membrane exocytosis protein 1 (Rab-3-interacting molecule 1) (RIM 1) (Rab-3-interacting protein 1). Gene symbol: RIMS1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q99NE5 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RIMS1 |
| Ontology domain: | Cellular Component |
| SynGO term: | extrinsic component of presynaptic active zone membrane (GO:0098891) |
| Synapse type(s): | hippocampus, GABAergic |
| Annotated paper: | de Jong APH, et al. "RIM C(2)B Domains Target Presynaptic Active Zone Functions to PIP(2)-Containing Membranes" Neuron. 2018 Apr 18;98(2):335-349.e7 PMID:29606581 |
| Figure(s): | Figure 1, 3, 4, 5 |
| Annotation description: | De Jong et. al demonstrate that RIM1 binds to the plasma membrane of the presynaptic active zone. - In STED microscopy, RIM colocalizes with Bassoon, a well established active zone marker. This colocalization is true for both endogenous RIM as well as RIM1 rescue constructs, which will be used in the remainder of the study (Figure 1 G-M) - The C2B domain binds to PIP2, a phospholipid that is present in the plasma membrane. The interaction is demonstrated with NMR spectroscopy, lipid cosedimentation and lipid cofloatation assays (Figure 3A-F). Additionally, a mutation is identified that abolishes PIP2 binding (Figure 4). - The PIP2-binding mutation in the C2B domain inhibits the function of RIM1 C2B in action-potential induced synaptic transmission (Figure 5). |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Super resolution Whole-cell patch clamp |
| Annotator(s): | Arthur de Jong (ORCID:0000-0002-7620-2704) Pascal Kaeser (ORCID:0000-0002-1558-1958) |
| Lab: | Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA |
| SynGO annotation ID: | 3958 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |