| Annotated protein: | RIMS-binding protein 2 (RIM-BP2). Gene symbol: RIMBP2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q80U40 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RIMBP2 |
| Ontology domain: | Biological Process |
| SynGO term: | voltage-gated ion channel activity involved in regulation of presynaptic membrane potential (GO:0099508) |
| Synapse type(s): | Calyx of Held |
| Annotated paper: | Sclip A, et al. "RIM-binding proteins recruit BK-channels to presynaptic release sites adjacent to voltage-gated Ca(2+)-channels" EMBO J. 2018 Aug 15;37(16):e98637 PMID:29967030 |
| Figure(s): | Figure 1, 4, 6, 8 |
| Annotation description: | Sclip et al provide multiple lines of evidence to demonstrate that RIMBP2 regulates BK-channels at the presynapse: - Using yeast-2-hybrid and immunoprecipitation it is shown that RIMBP2 interacts with BK-alpha. (Figure 1) -In HEK cells, RIMBP2 recruits BK-alpha to the plasma membrane (figure 1 D, E) and increases the BK-alpha mediated K+ current at lower membrane voltages (figure 4). -In line with the observations above, knockout of RIMBP1 and RIMBP2 (DKO) reduces the BK-mediated K+ current in the Calyx of Held (Figure 6A-H), and slow down the activation kinetics of these currents (Figure 6J-M). -The DKO also reduces the recruitment of BK-alpha to the presynaptic terminal (Figure 8). |
| Evidence tracking, Biological System: | Intact tissue Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Confocal Whole-cell patch clamp IP + WB/MSMS Y2H |
| Annotator(s): | Arthur de Jong (ORCID:0000-0002-7620-2704) Pascal Kaeser (ORCID:0000-0002-1558-1958) |
| Lab: | Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA |
| SynGO annotation ID: | 3956 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |