Annotated protein:cAMP-dependent protein kinase type I-beta regulatory subunit. Gene symbol: PRKAR1B. Taxonomy: Mus musculus (Mouse). Uniprot ID: P12849
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SynGO gene info:SynGO data @ PRKAR1B
Ontology domain:Cellular Component
SynGO term:postsynapse (GO:0098794)
Synapse type(s):hippocampus, glutamatergic
Annotated paper:Zhong H, et al. "Subcellular dynamics of type II PKA in neurons" Neuron. 2009 May 14;62(3):363-74 PMID:19447092
Figure(s):Figure 1, 2
Annotation description:Zhong et.al. use overexpression of GFP-tagged proteins to test the subcellular localization of PKA subunits in hippocampal slice cultures. They observe that PKA R1b-GFP is present in dendrites, including dendritic spines (Figure 2C, D).
It is also observed that PKA R1b-GFP expression is somewhat reduced in the axon. This indicates that this experiment has at least some specificity, but the evidence is not very strong.
Evidence tracking, Biological System:Intact tissue
Evidence tracking, Protein Targeting:Over-expression
Evidence tracking, Experiment Assay:Two-photon microscopy
Annotator(s):Arthur de Jong (ORCID:0000-0002-7620-2704)
Pascal Kaeser (ORCID:0000-0002-1558-1958)
Lab:Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA
SynGO annotation ID:3955
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology