| Annotated protein: | Disintegrin and metalloproteinase domain-containing protein 22 (ADAM 22). Gene symbol: ADAM22. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9R1V6 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ADAM22 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic density membrane (GO:0099061) |
| Synapse type(s): | brain, glutamatergic hippocampus, glutamatergic |
| Annotated paper: | Fukata Y, et al. "Epilepsy-related ligand/receptor complex LGI1 and ADAM22 regulate synaptic transmission" Science. 2006 Sep 22;313(5794):1792-5 PMID:16990550 |
| Figure(s): | Fig. 1, S3, S6 |
| Annotation description: | Fig. 1B shows that ADAM22 co-immunoprecipitate with the postsynaptic scaffold protein PSD-95 form adult rat brain extracts. Fig. S6 C and D show that overexpressed ADAM22-GFP colocalize with endogenpus PSD-95 at synapses. ADAM22-GFP mutant unables to bind PSD-95 do not localize to synapses. 5/4/2017 Pim Fig. S3: The figures above do not exclude a interaction of PSD-95 (postsynaptic marker) with a presynaptic ADAM22. In Fig.1 the interaction may be indirect and in Fig.S6 the resolution of the used technology is insufficient to distinguis pre- from post-synaptic localization. Fig. S3 demonstrates that PSD-95 interacts with ADAM22 directly, which supports a postsynaptic localization of ADAM22. - The "integral component of ..." is derived from the TM domains in ADAM22. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Biochemical fractionation (generic) IP + WB/MSMS |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| Additional literature: | Fig. 2C shows that ADAM22 is a transmembrane protein localized to dendrites because the extracellular domain of overexpressed GFP-ADAM22 interact with secreted LGI1 @ PMID:20133599 |
| SynGO annotation ID: | 395 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |