Annotated protein:AP-2 complex subunit sigma (Adaptor protein complex AP-2 subunit sigma) (Adaptor-related protein complex 2 subunit sigma) (Clathrin assembly protein 2 sigma small chain) (Clathrin coat assembly protein AP17) (Clathrin coat-associated protein AP17) (Plasma membrane adaptor AP-2 17 kDa protein) (Sigma-adaptin 3b) (Sigma2-adaptin). Gene symbol: AP2S1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P62744
antibody wiki:
SynGO gene info:SynGO data @ AP2S1
Ontology domain:Biological Process
SynGO term:postsynaptic neurotransmitter receptor endocytosis (GO:0098884)
Synapse type(s):cerebral cortex, glutamatergic
Annotated paper:Kastning K, et al. "Molecular determinants for the interaction between AMPA receptors and the clathrin adaptor complex AP-2" Proc Natl Acad Sci U S A. 2007 Feb 20;104(8):2991-6 PMID:17289840
Figure(s):Fig.1, 3, 4, 5
Annotation description:rule: NAS_CPLX_2
rationale:
The AP2 clathrin adapter is generally accepted to be a heterotetramer with subunit composition (AP2A1 OR AP2A2), AP2M1, AP2B1, AP2S1. Note that two gene paralogs are known for the alpha subunit (AP2A). Using an dominant negative peptide of the AP2M1 subunit the AP2 complex is shown to be involved in 'postsynaptic neurotransmitter receptor endocytosis (GO:0098884)'. Transfer of this annotation to the obligatory AP2B1 and AP2S1 subunitsis trivial based on the established protein composition of the AP2 complex.
P_ref BP:
[human AP2M1] SynGO ID 2859 (PMID:17289840)
P_inf CC:
[AP2S1_MOUSE] SynGO ID 3937 (PMID:25211037, proteomics)
Complex membership: AP2S1 protein is a fixed member of the AP2 clathrin adapter:
- Shown in crystal structure (PMID:26960121, see Fig.1+2). Literal excerpt: "AP2 is a large (300kDa) heterotetrameric complex comprising large α and β2 subunits, a medium μ2 subunit and a small σ2 subunit."
Evidence tracking, Biological System:Intact tissue
Cultured neurons
Non-neuronal tissue
Evidence tracking, Protein Targeting:Over-expression
Antagonist / agonist
Evidence tracking, Experiment Assay:Wide-field fluorescence
Whole-cell patch clamp
IP + WB/MSMS
Non-tracable Author Statement (NAS):This annotation required some inference over additional information/literature, and is not solely based on observations made in the annotated paper by its original authors.
Annotator(s):Pim van Nierop (ORCID:0000-0003-0593-3443)
Guus Smit (ORCID:0000-0002-2286-1587)
Matthijs Verhage (ORCID:0000-0002-2514-0216)
Lab:Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
SynGO annotation ID:3944
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology