Annotated protein:AP-2 complex subunit beta (AP105B) (Adaptor protein complex AP-2 subunit beta) (Adaptor-related protein complex 2 subunit beta) (Beta-2-adaptin) (Beta-adaptin) (Clathrin assembly protein complex 2 beta large chain) (Plasma membrane adaptor HA2/AP2 adaptin beta subunit). Gene symbol: AP2B1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P62944
antibody wiki:
SynGO gene info:SynGO data @ AP2B1
Ontology domain:Cellular Component
SynGO term:extrinsic component of presynaptic endocytic zone membrane (GO:0098894)
Synapse type(s):brain
Annotated paper:Takei K, et al. "The synaptic vesicle cycle: a single vesicle budding step involving clathrin and dynamin" J Cell Biol. 1996 Jun;133(6):1237-50 PMID:8682861
Figure(s):Fig. 2
Annotation description:In figure 2 The authors prepare synaptosomes (nerve terminals) that they treat with GTPgammaS to trap endocytic vesicles. They then use immunogold with an antibody against AP-2 alpha adaptin to demonstrate the protein at endocytic sites.

04/10/2018 Pim
The AP-2 adaptin is used a P_ref (reference protein) for protein complex based NAS annotation:

rule: NAS_CPLX_1
rationale:
The AP2 clathrin adapter is generally accepted to be a heterotetramer with subunit composition (AP2A1 OR AP2A2), AP2M1, AP2B1, AP2S1. Note that two gene paralogs are known for the alpha subunit (AP2A). Using an antibody against the AP2A subunit the AP2 complex is shown to be localized to the 'extrinsic component of presynaptic endocytic zone membrane (GO:0098894)'. Transfer of this annotation to the obligatory AP2M1, AP2B1 and AP2S1 subunits is trivial based on the established protein composition of the AP2 complex.
P_ref CC:
[rat Ap2a1 OR rat Ap2a2] SynGO ID 2541 (PMID:8682861, immunoEM) Note: this annotation was not accepted since data was not specific at gene paralog level.
P_inf CC:
[AP2B1_MOUSE] SynGO ID 3936 (PMID:25211037, proteomics)
Complex membership: AP2B1 protein is a fixed member of the AP2 clathrin adapter:
- Shown in crystal structure (PMID:26960121, see Fig.1+2). Literal excerpt: "AP2 is a large (300kDa) heterotetrameric complex comprising large α and β2 subunits, a medium μ2 subunit and a small σ2 subunit."
Evidence tracking, Biological System:Intact tissue
Evidence tracking, Protein Targeting:Antibody (detection)
Evidence tracking, Experiment Assay:Electron Microscopy
Non-tracable Author Statement (NAS):This annotation required some inference over additional information/literature, and is not solely based on observations made in the annotated paper by its original authors.
Annotator(s):Peter McPherson (ORCID:0000-0001-7806-5662)
Lab:Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, QC H3A 2B4, Canada
SynGO annotation ID:3940
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology