| Annotated protein: | Ephrin-B3. Gene symbol: EFNB3. Taxonomy: Mus musculus (Mouse). Uniprot ID: O35393 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ EFNB3 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of presynaptic membrane (GO:0099056) |
| Synapse type(s): | Mossy fiber synapse (DG->CA3) |
| Annotated paper: | Armstrong JN, et al. "B-ephrin reverse signaling is required for NMDA-independent long-term potentiation of mossy fibers in the hippocampus" J Neurosci. 2006 Mar 29;26(13):3474-81 PMID:16571754 |
| Figure(s): | Fig 1 |
| Annotation description: | Fig 1 Immunolabeling and confocal imaging of ephrinB3 shows that ephrinB3 is localized to mossy fiber axons and synaptic terminals in hippocampus of mice, specifically ephrin-B3-Beta-galactosidase fusion protein was colocalized with presynaptic marker synapsin 1 in stratum lucidum of CA3. 23/6/2017 Pim - Literal: "triple-immunofluorescent labeling and confocal microscopy confirmed that immunoreactivity for ephrin-B3-β-galactosidase fusion protein (red) was not colocalized with the postsynaptic dendritic marker MAP2 (green) (e, f) but was colocalized with the presynaptic marker synapsin I (green)" - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Tony Cijsouw (ORCID:0000-0003-0912-1514) Thomas Biederer (ORCID:0000-0002-3670-7863) |
| Lab: | Department of Neuroscience, Tufts University School of Medicine, Boston, MA 02111, USA |
| Additional literature: | Cloning of ephrinB3 (EPLG8) suggest a transmembrane containing protein. @ PMID:9126477 Fig1A,B An accumulation of VGluT1 signal was observed in axons crossing ephrinB3-expressing HEK293 cells, but not in those that contact GFP-expressing cells, suggesting that ephrinB3 is a transmembrane protein localized to the plasma membrane. @ PMID:17626212 Fig 1 and Fig 2. Through immunostaining and confocal imaging it was shown that EphrinB3 is coexpressed with Tau-1 and synaptobrevin in puncta and the growth cones of DIV4 and DIV4 hippocampal cultures, suggesting a pre-synpatic localization of ephrinB3 (besides a post-synaptic localization). @ PMID:16466709 |
| SynGO annotation ID: | 393 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |