| Annotated protein: | GTP-binding protein Rheb (Ras homolog enriched in brain). Gene symbol: RHEB. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q62639 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RHEB |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynapse organization (GO:0099175) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Yasuda S, et al. "Activation of Rheb, but not of mTORC1, impairs spine synapse morphogenesis in tuberous sclerosis complex" Sci Rep. 2014 Jun 3;4:5155 PMID:24889507 |
| Figure(s): | Fig 3 |
| Annotation description: | Rat cultured hippocampal neurons transfected at DIV19 with FLAG-RhebQ64V (Inactive RHEB) completely abolished spine synapse formation in WT neurons. 18/01/2021 Dnyanada : Tuberous sclerosis complex (TSC) is a systemic genetic disease that demonstrates impaired spine formation (Figure 1). The paper investigates the role of RHEB, a GTPase located downstream of the TSC 1/2 complex in the impaired spine formation phenotype. Expression of active Rheb (FLAG-RhebQ64V ) abolishes dendritic spine formation in WT neurons, whereas inactive Rheb (FLAG-RhebD60I) restores spine synapse formation in Tsc2+/- neurons. Furthermore, inactivation of Rheb with farnesyltransferase inhibitors recoveres spine synapse morphogenesis in Tsc2+/- neurons. Based on these observations on spine formation, the term "regulation of postsynapse organization" was selected. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Alexandros Kanellopoulos (ORCID:0000-0002-2094-7491) Vittoria Mariano (ORCID:0000-0002-9848-0262) Achsel Tilmann (ORCID:0000-0002-1190-4481) Claudia Bagni (ORCID:0000-0002-4419-210X) |
| Lab: | Department of Fundamental Neurosciences, University of Lausanne, CH-1006 Lausanne, Switzerland; Dept Biomedicine and Prevention, University of Rome Tor Vergata, 00133 Rome, Italy |
| Additional literature: | In drosophila melanogaster impaired levels of RHEB specifically in neurons (NMJ) induce increase synapses (fig 1). Numbers of synaptic boutons/muscle are increased upon RHEB Overexpression. Neuron-specific expression of Rheb also produced electrophysiological changes at the NMJ, determined by intracellular recordings from abdominal muscle 6 in third instar larvae. The amplitude of the EJP was significantly increased in animals overexpressing Rheb compared to controls. Quantal content, a measure of the number of synaptic quanta released in a single firing of the motoneuron, was nearly doubled by neuron-directed expression of Rheb compared to controls. Mini-EJP amplitude was decreased upon RHEB over expression. @ PMID:17440611 |
| SynGO annotation ID: | 3912 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |