| Annotated protein: | Ephrin-B2 (ELF-2) (EPH-related receptor tyrosine kinase ligand 5) (LERK-5) (HTK ligand) (HTK-L). Gene symbol: EFNB2. Taxonomy: Mus musculus (Mouse). Uniprot ID: P52800 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ EFNB2 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic density membrane (GO:0099061) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Grunwald IC, et al. "Hippocampal plasticity requires postsynaptic ephrinBs" Nat Neurosci. 2004 Jan;7(1):33-40 PMID:14699416 |
| Figure(s): | Fig. 1 |
| Annotation description: | Fig. 1 CA1-region synapses immunolabeled with ephrinB2 antibody and analyzed by electron microscopy indicate ephrinB2 expression at postsynaptic sites. 23/6/2017 Pim - CA1 neurons express the ephrinB2 protein consistent with postsynaptic localization. - 30% of gold particles are on the presynaptic compartment - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Tony Cijsouw (ORCID:0000-0003-0912-1514) Thomas Biederer (ORCID:0000-0002-3670-7863) |
| Lab: | Department of Neuroscience, Tufts University School of Medicine, Boston, MA 02111, USA |
| Additional literature: | Fig 1 CA1-region synapses immunolabeled with ephrinB2 antibody and analyzed by EM found that labeling was predominantly at or close to postsynaptic density of synapses. @ PMID:14699416 Cloning of mouse and human EFNB2 (HTKL) suggests a transmembrane domain containing protein and protein expression in COS cells reveals surface expression of ephrinB2 @ PMID:7534404 |
| SynGO annotation ID: | 391 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |