| Annotated protein: | Ephrin B2. Gene symbol: EFNB2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: A6IWT6 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ EFNB2 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of presynaptic membrane (GO:0099056) |
| Synapse type(s): | neocortex, glutamatergic |
| Annotated paper: | McClelland AC, et al. "Ephrin-B1 and ephrin-B2 mediate EphB-dependent presynaptic development via syntenin-1" Proc Natl Acad Sci U S A. 2009 Dec 1;106(48):20487-92 PMID:19915143 |
| Figure(s): | Fig. 3 |
| Annotation description: | Fig 3 shows that in confocal images of rat cultured neurons antibody-stained ephrinB2 colocalizes with antibody-stained vGlut1. 22/6/2017 Pim - Authors go great length showing presynaptic localization (Fig.3) and show that presynapse-selective KD of the protein interferes with synapse formation. - TM localization is not shown in this paper. However, the protein has TM domains ans in known to localize to the plasma membrane (see Additional literature), and mediates responses to its postsynaptic ligand (EphB2) expressed on HEK cells (this paper). Based on these observation I approve to IC the term not literally shown in the paper. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Tony Cijsouw (ORCID:0000-0003-0912-1514) Thomas Biederer (ORCID:0000-0002-3670-7863) |
| Lab: | Department of Neuroscience, Tufts University School of Medicine, Boston, MA 02111, USA |
| Additional literature: | Cloning of mouse and human EFNB2 (HTKL) suggests a transmembrane domain containing protein and protein expression in COS cells reveals surface expression of ephrinB2 @ PMID:7534404 |
| SynGO annotation ID: | 390 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |