| Annotated protein: | Poly(A) RNA polymerase GLD2 (EC 2.7.7.19) (PAP-associated domain-containing protein 4) (Terminal nucleotidyltransferase 2). Gene symbol: TENT2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q5U315 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ TENT2 |
| Ontology domain: | Biological Process |
| SynGO term: | RNA polyadenylation at postsynapse (GO:0140235) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Udagawa T, et al. "Bidirectional control of mRNA translation and synaptic plasticity by the cytoplasmic polyadenylation complex" Mol Cell. 2012 Jul 27;47(2):253-66 PMID:22727665 |
| Figure(s): | Fig.2, 4 |
| Annotation description: | Fig.2: "Gld2 regulates basal and NMDA-induced dendritic poly(A)" - "NMDA treatment (100 nM, 30 seconds) increased dendritic oligo(dT) FISH intensity by 55% in distal regions as compared to control (Figure 2B), which was abrogated by the NMDAR antagonist APV (data not shown). NMDA did not affect dendritic αCaMKII mRNA levels (Figure 2B,S2B), indicating negligible transcript transport to distal dendrites during the brief stimulation. To determine if dendritic polyadenylation was sensitive to Gld2, neurons were transduced with lentiviruses expressing Gld2 shRNA or a control (Figure S5). Depletion of Gld2 reduced dendritic oligo(dT) FISH signals relative to controls (Figure 2C,D), indicating that this enzyme regulates dendritic polyadenylation." Fig.4: "Identification of mRNAs whose polyadenylation is controlled by Gld2" - "The data presented above imply that Gld2 controls dendritic polyadenylation and translation of specific mRNAs. To identify them, RNA was extracted from hippocampal neurons transduced with Gld2 shRNA-expressing or control lentivirus...Twenty-seven of these mRNAs have been implicated in synaptic plasticity and/or nervous system disorders; five were selected and the microarray data were validated by qRTPCR (Figure 4C). HuD (RNA binding protein) and Neto2 (kainate receptor modulator) mRNAs were reduced in the total and poly(A) RNA fractions following Gld2 knockdown, suggesting that they became unstable or their transcription was indirectly reduced upon Gld2 depletion. NR2A (NMDAR subunit) and Ago3 (Piwi protein) mRNAs were reduced only in the poly(A) RNA fraction, suggesting that their poly(A) tails were shortened by Gld2 depletion without affecting stability..." 9/7/2018 Pim - The use of NMDA supports the synaptic context of the observed phenomenon. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Fluorescence in situ hybridization (FISH) |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 3871 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |