| Annotated protein: | Poly(A)-specific ribonuclease PARN (EC 3.1.13.4) (Polyadenylate-specific ribonuclease). Gene symbol: PARN. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: A0A0G2K6N8 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PARN |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Udagawa T, et al. "Bidirectional control of mRNA translation and synaptic plasticity by the cytoplasmic polyadenylation complex" Mol Cell. 2012 Jul 27;47(2):253-66 PMID:22727665 |
| Figure(s): | Fig.1 |
| Annotation description: | Fig.1: "Interaction and co-localization of CPEB-containing cytoplasmic complex proteins" - "To assess whether these components were co-localized in dendrites, cultured neurons were coimmunostained for symplekin and each other factor and 3D deconvolved images were analyzed (Figure 1G). CPEB, PARN, Gld2 and Ngd were non-randomly co-localized with symplekin (p > 0.95), while GluR1 was not (Figure 1H). " - "These proteins were also detected in dendritic spines; 3D reconstructions of phalloidin fluorescence (Figure 1J) showed that 23.1% ± 1.24% of spines contained symplekin and 80.1% ± 2.54% of symplekin-positive spines also contained CPEB, Gld2, PARN, or Ngd immunoreactivity (n=40 cells, 1196 spines). These data indicate that the cytoplasmic polyadenylation machinery forms complexes in dendrites and at synapses." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 3869 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |