| Annotated protein: | Lysophosphatidic acid receptor 1 (LPA receptor 1) (LPA-1) (Lysophosphatidic acid receptor Edg-2). Gene symbol: LPAR1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P61794 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ LPAR1 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic membrane (GO:0099055) |
| Synapse type(s): | medulla, GABAergic |
| Annotated paper: | Garcia-Morales V, et al. "Membrane-derived phospholipids control synaptic neurotransmission and plasticity" PLoS Biol. 2015 May 21;13(5):e1002153 PMID:25996636 |
| Figure(s): | Fig.1, 6 |
| Annotation description: | Fig.1: "Expression pattern of LPA1 in the HN supports a role for lysophospholipids in the control of synaptic neurotransmission" - "Three-dimensional reconstructions agreed with a cytoplasmic and membrane localization of LPA1 in perikarya and main dendritic branches of HMNs (Fig 1E and 1F). Triple immunofluorescence for LPA1, SMI32, and the vesicular glutamate (VGLUT2) or GABA (VGAT) transporters as synaptic markers confirmed that LPA1-ir puncta were colocalizing with excitatory (VGLUT2-ir) or inhibitory (VGAT-ir) presynaptic structures (Fig 1G, 1H, 1J, and 1K). Both excitatory and inhibitory inputs were also found apposed to SMI32-ir neuropil or somata coexpressing LPA1 (Fig 1H and 1I). Although LPA1 expression in other neural cell types is not excluded, this expression pattern supports pre and/or postsynaptic roles of LPA1 at the main excitatory and inhibitory inputs on HMNs, suggesting a potential contribution of LPA to motoneuron physiology." Fig.6: "LPA induces GABAAergic STD and GABAAγ2 dephosphorylation via postsynaptic LPA1-RhoA/ROCK-CaN signaling" - "Multiple immunolabeling confocal images of the HN from P7 rats showing colocalization between LPA1-ir and gephyrin-ir (top). 3-D reconstruction (bottom) showing that LPA1-ir colocalizes with gephyrin in a SMI32-ir HMN." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 3864 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |