| Annotated protein: | Voltage-dependent R-type calcium channel subunit alpha-1E (Brain calcium channel II) (BII) (Calcium channel, L type, alpha-1 polypeptide, isoform 6) (Voltage-gated calcium channel subunit alpha Cav2.3). Gene symbol: CACNA1E. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q61290 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CACNA1E |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of presynaptic membrane (GO:0099056) |
| Synapse type(s): | hippocampus midbrain |
| Annotated paper: | Parajuli LK, et al. "Quantitative regional and ultrastructural localization of the Ca(v)2.3 subunit of R-type calcium channel in mouse brain" J Neurosci. 2012 Sep 26;32(39):13555-67 PMID:23015445 |
| Figure(s): | Fig2 E-K, Fig3, Fig 4B, D |
| Annotation description: | Serial section analysis of electron microscopic images from the hippocampal CA1 showed Cav2.3 to be predominantly presynaptic in the interpeduncular nucleus but mainly expressed postsynaptically in other brain regions. Cav2.3 immunogold particles were observed primarily in the extrasynaptic plasma membrane and occasionally over the presynaptic active zone. The following characteristics were used to identify presynaptic terminals: synaptic vesicle accumulation, presence of presynaptic specializations, and rigid alignment of presynaptic membrane to its postsynaptic partner. Synapses were identified based on postsynaptic thickening and electron dense materials in the synaptic cleft. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Ryan J. Farrell (ORCID:0000-0003-4022-8707) Ghazaleh Ashrafi (ORCID:0000-0001-7480-0826) Camila Pulido (ORCID:0000-0002-5648-066X) Jaime de Juan-Sanz (ORCID:0000-0002-1212-5623) Timothy Ryan (ORCID:0000-0003-2533-9548) |
| Lab: | Department of Biochemistry, Weill Cornell Medicine, New York, NY 10065, USA |
| SynGO annotation ID: | 386 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |