| Annotated protein: | Neuropilin and tolloid-like protein 2 (Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 2). Gene symbol: NETO2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: C6K2K4 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NETO2 |
| Ontology domain: | Biological Process |
| SynGO term: | neurotransmitter receptor localization to postsynaptic specialization membrane (GO:0099645) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Sheng N, et al. "Neto auxiliary proteins control both the trafficking and biophysical properties of the kainate receptor GluK1" Elife. 2015 Dec 31;4:e11682 PMID:26720915 |
| Figure(s): | Fig.2, 5, 8 |
| Annotation description: | Fig.2: "Neto1 and Neto2 promote GluK1 receptor synaptic targeting" - Supplement 1; "Neto1 and Neto2 regulation of GluK1 synaptic expression is independent of synaptic activity." - Supplement 2; "NMDAR EPSCs are increased in GluK1/Neto1 and GluK1/Neto2 expressing neurons" Fig.5: "Neto2 specifically targets GluK1 receptors to silent synapse." Fig.8: "Neto1 and Neto2 increase GluK1 receptor surface trafficking and biophysical properties." - "As it has been reported that Neto2 increases GluK1 surface expression (Copits et al., 2011), Neto proteins may simply increase the pool of surface receptors to such an extent that the receptors passively populate synapses..." - "We then examined the Neto mutants that greatly impaired synaptic responses and looked for the Neto mutants to modulate KAR surface expression. If there were an additional targeting role for Netos we would expect some of these mutants, which impaired GluK1-mediated synaptic responses, to generate extrasynaptic KAR currents similar in magnitude to that recorded when wild type Netos were expressed with GluK1. Indeed, both Neto1S3Y/A and Neto2S4T/A mutants generated currents in outside-out patches of similar size to those generated by wild type Neto1 and Neto2 (Figure 8A). These results provide strong evidence that there is, in fact, a role for these auxiliary proteins in targeting surface GluK1 receptors to synapses." - Supplement 1: "GluK1 receptor is localized at synapse." |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal Whole-cell patch clamp |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 3859 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |