| Annotated protein: | Integrin subunit alpha 5. Gene symbol: ITGA5. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: A0A0G2K1E2 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ITGA5 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic membrane (GO:0099055) |
| Synapse type(s): | cerebellum, glutamatergic |
| Annotated paper: | Heintz TG, et al. "Regional Regulation of Purkinje Cell Dendritic Spines by Integrins and Eph/Ephrins" PLoS One. 2016 Aug 12;11(8):e0158558 PMID:27518800 |
| Figure(s): | Fig.1 |
| Annotation description: | Fig.1: "at DIV21, mature Purkinje cells were positive for both α3 and α5 integrin. α 3 puncta were present on both soma, dendritic shaft and on a subset of spines but with no particular pattern of distribution (Fig 1B). Similarly, staining for integrin α5 was scattered homogeneously over the entire cell, but was prominent on dendritic spines identified by proximity to puncta of bassoon staining (Fig 1A). Integrin β4 staining was also seen on a subset of spines scattered over the dendrites (Fig 1C). Our results suggest that in conditions of mixed cerebellar culture deprived of climbing fibres, integrins are homogeneously distributed over the dendritic tree of Purkinje cells. In order to verify that integrins are present in the postsynaptic regions, we performed a subcellular fractionation of cerebellar cultures according to the method of [27], and found β1 integrin enriched in the synaptoneurosome fraction." 3/7/2018 Pim - Localization to the postsynaptic membrane is derived from the TM domain present in integrin protein and the effect of exposure to peptides applied in the culture medium. - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 3845 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |