| Annotated protein: | Neuropilin and tolloid-like protein 1 (Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1). Gene symbol: NETO1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8R4I7 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NETO1 |
| Ontology domain: | Biological Process |
| SynGO term: | anterograde axonal transport of neurotransmitter receptor complex (GO:0140231) |
| Synapse type(s): | Schaffer collateral synapse (CA3->CA1) |
| Annotated paper: | Orav E, et al. "NETO1 Guides Development of Glutamatergic Connectivity in the Hippocampus by Regulating Axonal Kainate Receptors" eNeuro. 2017 Jul 3;4(3):ENEURO.0048-17.2017 PMID:28680963 |
| Figure(s): | Fig.3, 4, 5 |
| Annotation description: | Fig.3: "Loss of NETO1 leads to impaired GluK1c delivery to distal axons" Fig.4: "NETO1 is required for tonic activity of KARs at CA3-CA1 synapse in the neonatal hippocampus" Fig.5: "NETO1 deficiency leads to loss of functional presynaptic KARs at immature CA3-CA1 synapses that is rescued with GluK1c overexpression in the area CA3" 9/7/2018 Pim - The data shows that there are less KARs at the presynaptic membrane (ePhys). Since the authors claim that the trafficking of the KARs into the axon is perturbed in Neto1-/- neurons, I have considered the reduced amounts of the NT receptor at the presynaptic membrane (term 'regulation of presynaptic membrane neurotransmitter receptor levels') as secondary and I decided not to make this annotation for Neto1. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Confocal Whole-cell patch clamp |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 3843 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |