| Annotated protein: | Neuropilin and tolloid-like protein 2 (Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 2). Gene symbol: NETO2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: C6K2K4 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NETO2 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of neurotransmitter receptor localization to postsynaptic specialization membrane (GO:0098696) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Lomash RM, et al. "Phosphorylation of the kainate receptor (KAR) auxiliary subunit Neto2 at serine 409 regulates synaptic targeting of the KAR subunit GluK1" J Biol Chem. 2017 Sep 15;292(37):15369-15377 PMID:28717010 |
| Figure(s): | Fig.6 |
| Annotation description: | Fig.6: "Neto2 Ser-409 phosphorylation inhibits synaptic targeting of GluK1 in CA1 pyramidal neurons" - "As previously reported, we found a substantial increase in GluK1 synaptic responses in the presence of Neto2 WT (Fig. 6A). Co-expression of GluK1 with the phosphodeficient mutant Neto2 S409A also showed a similar increase in synaptic responses (Fig. 6B). By contrast, there was significantly less enhancement of synaptic currents when co-expressed with Neto2 S409D, the phosphomimetic mutant (Fig. 6, C and D). We have previously shown that Neto2 itself does not regulate AMPAR-mediated synaptic transmission (9). Taken together, these results show that when Neto2 is phosphorylated at Ser-409 the synaptic targeting of GluK1 is inhibited." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 3840 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |