| Annotated protein: | Huntingtin-interacting protein 1 (HIP-1) (Huntingtin-interacting protein I) (HIP-I). Gene symbol: HIP1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8VD75 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ HIP1 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic neurotransmitter receptor endocytosis (GO:0099149) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Metzler M, et al. "Disruption of the endocytic protein HIP1 results in neurological deficits and decreased AMPA receptor trafficking" EMBO J. 2003 Jul 1;22(13):3254-66 PMID:12839988 |
| Figure(s): | Fig.7, 8 |
| Annotation description: | Fig.7: "HIP1 colocalizes and interacts with GluR1" - "Cortical neurons were treated with 100 uM AMPA for 10 min or left untreated and the resulting change in the percentage of cell surface-expressed GluR2-containing AMPA receptors was determined for each genotype. In unstimulated neurons, ~60% of GluR2-containing AMPA receptors are expressed on the cell surface. This percentage is reduced to 37% following AMPA stimulation. In contrast, surface expression of GluR2 in neurons from HIP1-/- mice remained constant suggesting a block in AMPA receptor internalization. " Fig.8: "AMPA receptor internalization is decreased in primary neurons of HIP1-/- mice" - "Under control conditions (saline alone) wild-type neurons showed little constitutive GluR1 internalization, as indicated by the weak red staining. However, wild-type neurons showed a dramatic increase in GluR1 internalization after glutamate treatment, as the intensity of green cell surface staining decreased while the intensity of red staining in cell bodies increased. Again, this effect was markedly inhibited in HIP1-/- neurons where most of the staining remained associated with cell surface-expressed GluR1 receptors after glutamate stimulation, similar to cultures exposed to salt solution alone (control). Quantification of this result showed a highly significant difference in response to glutamate for HIP1-/- neurons (n = 3 independent experiments, P < 0.01 compared with wild type) demonstrating that glutamate-stimulated GluR1 internalization is blocked in neurons from HIP1-/- mice. " |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Optical physiology IP + WB/MSMS |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 3839 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |