| Annotated protein: | ArfGAP with GTPase domain, ankyrin repeat and PH domain 3. Gene symbol: AGAP3. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: U6A022 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ AGAP3 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | cerebral cortex, glutamatergic |
| Annotated paper: | Oku Y, et al. "AGAP3 and Arf6 regulate trafficking of AMPA receptors and synaptic plasticity" J Neurosci. 2013 Jul 31;33(31):12586-98 PMID:23904596 |
| Figure(s): | Fig.2 |
| Annotation description: | Fig.2: "AGAP3 is found at postsynaptic sites in the adult brain" - "Using antibodies targeted against either AGAP3 N terminus or C terminus, we were able to detect all three splice variants of AGAP3. AGAP3 and CRAG were both enriched in the PSDI, II, and III fractions" - "To confirm that AGAP3 is present at postsynaptic sites as shown in the subcellular fractionation blot, endogenous staining was done in Banker cultures at 21 DIV using the AGAP3 N-terminal antibody. Anti-AGAP3 signal overlapped primarily with the postsynaptic marker PSD95 rather than the presynaptic marker vGlut, suggesting that AGAP3 and CRAG have a postsynaptic localization (Fig. 2E). Specificity of AGAP3 staining was confirmed by loss of signal with transfection of shRNA#2 to knock down the expression of AGAP3 in dissociated hippocampal cultures (Fig. 2F). Together, these data suggest that AGAP3 is present at postsynaptic sites in the adult brain." [Fig.3: "AGAP3 is a component of the NMDA receptor signaling complex"] [Fig.6: "AGAP3 regulates AMPA receptor trafficking through the ArfGAP domain" - "We also looked at surface/total GluA1 levels with overexpression of WT, GTPase mutant, and ArfGAP mutant and observed similar results (data not shown). In contrast to AMPA receptor staining, we did not observe statistically significant differences in surface NMDA receptor subunit NR2A levels between all conditions (Fig. 6C). Furthermore, increases in surface GluA1 observed with loss of AGAP3 expression correlated with the increased spine density (Figs. 6A, ,77A;"] [Fig.7: "AGAP3 and Arf6 together regulate trafficking of GluA1"] [Fig.8: "AGAP3 regulates trafficking of GluA1 during chemical LTP"] |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 3835 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |