| Annotated protein: | Glutamate receptor 3 (GluR-3) (AMPA-selective glutamate receptor 3) (GluR-C) (GluR-K3) (Glutamate receptor ionotropic, AMPA 3) (GluA3). Gene symbol: GRIA3. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P19492 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GRIA3 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of presynaptic active zone membrane (GO:0099059) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Moga DE, et al. "Glutamate receptor subunit 3 (GluR3) immunoreactivity delineates a subpopulation of parvalbumin-containing interneurons in the rat hippocampus" J Comp Neurol. 2003 Jul 14;462(1):15-28 PMID:12761821 |
| Figure(s): | Fig.9 |
| Annotation description: | Fig.9: "Electron micrographs of CA1 rat hippocampal sections demonstrating immunogold labeling for GluR3 in asymmetric axospinous synapses." - Literal: "CA1 neuropil displayed asymmetric synapses immunoreactive for GluR3, which was localized throughout both the postsynaptic density (PSD) and the synaptic cleft (Fig. 9)." - "very few present in the spine (SP) or excitatory axon terminal (asterisk)" Note: "The specificity of MAb:3B3 for GluR3 in brain tissue sections was demonstrated by preincubating MAb:3B3 with the GluR1, GluR2, and GluR3 fusion proteins. The GluR3 fusion protein entirely blocked the immunoreactivity of MAb:3B3 in cortical sections (Fig. 2; only CA1 shown), while this immunoreactivity was not altered with the GluR1 and GluR2 fusion proteins compared to controls in which the fusion proteins were omitted." - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 3834 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |