| Annotated protein: | E3 ubiquitin-protein ligase parkin (EC 2.3.2.31) (Parkin RBR E3 ubiquitin-protein ligase). Gene symbol: PRKN. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9JK66 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PRKN |
| Ontology domain: | Cellular Component |
| SynGO term: | synaptic vesicle membrane (GO:0030672) |
| Synapse type(s): | brainstem thalamus midbrain |
| Annotated paper: | Mouatt-Prigent A, et al. "Ultrastructural localization of parkin in the rat brainstem, thalamus and basal ganglia" J Neural Transm (Vienna). 2004 Oct;111(10-11):1209-18 PMID:15480834 |
| Figure(s): | Fig.3 |
| Annotation description: | Fig.3: "Parkin immunoreactivity in neurons" - "Parkin immunoreactivity was also detected in dendrites (arrows in B) and nerve terminals (arrowhead in B) at the level a symmetric synapse on the upper part of the nerve terminal. Within these cellular compartments, Parkin immunoreactivity was also associated with synaptic vesicles (arrow in B), where the staining appeared weaker, however. Furthermore, not all nerve terminals were Parkin immunopositive." - "In Parkin-immunostained terminals, labeling was present in the pre- and post-synaptic elements of the synapse (Fig. 3B). Yet, staining intensity was lower in pre-synaptic elements than in post-synaptic elements." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 3832 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |