| Annotated protein: | Voltage-dependent N-type calcium channel subunit alpha-1B (CHCACHA1B) (Voltage-gated calcium channel subunit alpha Cav2.2). Gene symbol: CACNA1B. Taxonomy: Gallus gallus (Chicken). Uniprot ID: O73706 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CACNA1B |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of presynaptic membrane (GO:0099056) |
| Synapse type(s): | Calyx of Held |
| Annotated paper: | Haydon PG, et al. "Localization of individual calcium channels at the release face of a presynaptic nerve terminal" Neuron. 1994 Dec;13(6):1275-80 PMID:7993621 |
| Figure(s): | Fig 1, 2, 4B |
| Annotation description: | Fig 1, 2. Used atomic force microscopy (AFM) on the calyx-type nerve terminal of the chick ciliary ganglion to localize single calcium channels. Calcium channels were tagged using a biotylinated form of the Cav2.2 specific venom peptide omega-conotoxin GVIA (b-omega-CTx). The labelled channels were visualized using avidin-coated 30nm gold particles. Controls used: untreated, b-omega-CTx treated without gold, and preblocking with untagged omega-CTx. Nerve terminals were identified in vitro from structural clues together with staining using the vital dye 4-Di-2-Asp The location of the terminals was noted by their relation to an electron microscopy grid attached to the underside of the coverslip. A sketch was also made of the terminal and the surrounding cells and cell debris so that the calyx could be re-identified under the AFM. 30/6/2017 Pim - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Super resolution |
| Annotator(s): | Ryan J. Farrell (ORCID:0000-0003-4022-8707) Ghazaleh Ashrafi (ORCID:0000-0001-7480-0826) Camila Pulido (ORCID:0000-0002-5648-066X) Jaime de Juan-Sanz (ORCID:0000-0002-1212-5623) Timothy Ryan (ORCID:0000-0003-2533-9548) |
| Lab: | Department of Biochemistry, Weill Cornell Medicine, New York, NY 10065, USA |
| Additional literature: | Peptides corresponding to a highly variable segment in the intracellular loop between domains II and III of the alpha1 subunit of brain calcium channels were synthesized, coupled to BSA as a carrier and used as an antigen for antibody production. The site directed anti-peptide antibody for Cav2.2 was used to stain sections of adult rat brain. Punctate staining that resembled nerve terminals was observed along cell bodies and dendrites of cortical and hippocampal pyramidal neurons, on cerebellar Purkinje cells, and on the dendrites of many other classes of neurons (Fig 7). The antibody also labelled the large terminals at the synapse between the mossy fibers of the dentate granule neurons and hippocampal CA3 pyramidal cells (Fig 4E). @ PMID:1334419 Tetramethylrhodamine-conjugated omega-conotoxin was used as a fluorescent stain to determine the spatial distribution of voltage-gated Ca2+ channels along frog motor nerve terminals (Fig 1). The fluorescent toxin blocked neuromuscular transmission irreversibly, was confined to the neuromuscular junction and labeling was prevented by pretreatment with omega-conotoxin and by prior denervation for 5-7 d. Combined fluorescence and phase-contrast optics indicated that the stain was on the synaptic rather than the nonsynaptic side of the nerve terminal (Fig 4) and that the bands and dots of the stain pattern were in spatial register with postsynaptic junctional folds (Fig 5). @ PMID:1707093 |
| SynGO annotation ID: | 383 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |