| Annotated protein: | Integrin alpha-3 (CD49 antigen-like family member C) (Galactoprotein B3) (GAPB3) (VLA-3 subunit alpha) (CD antigen CD49c) [Cleaved into: Integrin alpha-3 heavy chain; Integrin alpha-3 light chain]. Gene symbol: ITGA3. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q62470 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ITGA3 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of presynaptic active zone membrane (GO:0099059) |
| Synapse type(s): | Neuro-muscular junction |
| Annotated paper: | Ross JA, et al. "Multiple roles of integrin-alpha3 at the neuromuscular junction" J Cell Sci. 2017 May 15;130(10):1772-1784 PMID:28386022 |
| Figure(s): | Fig.1 |
| Annotation description: | Fig.1: "Localisation of integrin-α3 at the AZs of NMJs" - "To determine the localisation of integrin-α3 at the mouse NMJ, we stained longitudinal sections of sternomastoid muscle from embryonic day (E)18.5 mice with antibodies to integrin-α3 and with fluorescently conjugated α-bungarotoxin to mark postsynaptic AChR clusters (Fig. 1). We found a clear localisation of integrin-α3 apposed to the AChR plaque (Fig. 1A). Imaging at high magnification revealed that integrin-α3 was specifically concentrated at AZs, colocalizing with bassoon-positive puncta (Fig. 1B); this was verified using line scans across the synapse to measure fluorescence intensities and spatial distribution of integrin-α3, bassoon and AChRs (Fig. 1E)." - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 3806 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |