| Annotated protein: | Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1 (Brain cyclic nucleotide-gated channel 1) (BCNG-1) (Hyperpolarization-activated cation channel 2) (HAC-2). Gene symbol: HCN1. Taxonomy: Mus musculus (Mouse). Uniprot ID: O88704 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ HCN1 |
| Ontology domain: | Biological Process |
| SynGO term: | intracellular cAMP-activated cation channel activity involved in regulation of presynaptic membrane potential (GO:0140232) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Huang Z, et al. "Presynaptic HCN1 channels regulate Cav3.2 activity and neurotransmission at select cortical synapses" Nat Neurosci. 2011 Apr;14(4):478-86 PMID:21358644 |
| Figure(s): | Fig.5, 7 |
| Annotation description: | Fig.5: "HCN1 channels inhibit mEPSC frequency by limiting T-type Ca2+ channel activity" [Fig.6: "Co-localisation of HCN1 and CaV3.2 subunits on EC synaptic terminals"] Fig.7: "Hyperpolarization enhances pre-synaptic T-type Ca2+ channel activation" - regulation of presynaptic membrane potiential literal: "The significant findings of this study are that functional HCN1 channels are present on glutamatergic synaptic terminals synapsing onto EC layer III neurons where they reduce spontaneous and evoked neurotransmitter release. These effects are at least partly due to altered availability of low-threshold, voltage-gated T-type Ca2+ channel. In support, electron microscopy showed that HCN1 and CaV3.2 subunits co-exist on EC pre-synaptic terminals...Remarkably, our results suggest that the presence of pre-synaptic HCN channels depolarizes the RMP, thereby restricting Ca2+ entry via pre-synaptic T-type Ca2+ channel activity and preventing glutamate release (Figs 44 -7). This suggests that pre-synaptic HCN channels may provide an additional regulatory mechanism for controlling synaptic release at these synapses." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 3800 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |