| Annotated protein: | Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1 (Brain cyclic nucleotide-gated channel 1) (BCNG-1) (Hyperpolarization-activated cation channel 2) (HAC-2). Gene symbol: HCN1. Taxonomy: Mus musculus (Mouse). Uniprot ID: O88704 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ HCN1 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of presynaptic active zone membrane (GO:0099059) |
| Synapse type(s): | neocortex |
| Annotated paper: | Huang Z, et al. "Presynaptic HCN1 channels regulate Cav3.2 activity and neurotransmission at select cortical synapses" Nat Neurosci. 2011 Apr;14(4):478-86 PMID:21358644 |
| Figure(s): | Fig.3 |
| Annotation description: | Fig.3: - "Immunoparticles for HCN1 along the extrasynaptic plasma membrane (e.g. arrows) of dendritic spines (s) and shafts (Den) establishing synapses with excitatory terminals" - "Pre-synaptic HCN1 subunits in excitatory terminals were mainly located in the active zone (b)" literal: "To determine if HCN1 subunits are located on EC synaptic terminals, we carried out electron microscopy studies using a pre-embedding immunogold method (see Methods). We counted 2752 particles in the medial EC from sections obtained from 3 wildtype mice. 72.2 ± 0.8% of HCN1 immunogold particles were observed along the extra-synaptic plasma membrane of dendritic shafts and spines (Fig 3a, e), confirming that HCN channels are largely present in EC dendrites. Interestingly, 27.8 ± 1.0% of immunogold particles were located at pre-synaptic sites (Fig 3b, e)." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | similar EM data but of less quality @ PMID:17460082 |
| SynGO annotation ID: | 3799 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |