| Annotated protein: | Syntaxin-4. Gene symbol: STX4. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q08850 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ STX4 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic membrane (GO:0099055) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Kennedy MJ, et al. "Syntaxin-4 defines a domain for activity-dependent exocytosis in dendritic spines" Cell. 2010 Apr 30;141(3):524-35 PMID:20434989 |
| Figure(s): | Fig.4 |
| Annotation description: | Fig.4: "Syntaxin 4 Localizes to Lateral Spine Domains" - "To determine whether endogenous Stx4 resides postsynaptically in vivo, we performed immunogold electron microscopy (EM) focusing on stratum radiatum of area CA1 of rat hippocampus, using antibodies against all plasma membrane syntaxins (1-4). Stx4 label was often found near the spine membrane at sites lateral to the PSD (Figures 4E and 4F). Occasional labeling was observed presynaptically, but quantitative analysis revealed a 4-fold postsynaptic enrichment of Stx4 (Figures 4F and 4G). In contrast, Stx1 immunogold labeling was enriched 2-fold in presynaptic compartments, while Stx2 and Stx3 were nearly evenly distributed between the pre and postsynaptic compartments (Figures (Figures4G4G and S2C). Analysis of the tangential distribution of postsynaptic Stx4 signal along the spine PM revealed a strong peak of labeling in lateral domains (Figure 4H) consistent with Stx4 enrichment just outside of the PSD." 19/6/2018 Pim - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Ruud Toonen (ORCID:0000-0002-9900-4233) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 3794 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |