| Annotated protein: | E3 ubiquitin-protein ligase parkin (EC 2.3.2.31) (Parkin RBR E3 ubiquitin-protein ligase). Gene symbol: PRKN. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9WVS6 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PRKN |
| Ontology domain: | Biological Process |
| SynGO term: | regulation protein catabolic process at presynapse (GO:0140251) |
| Synapse type(s): | striatum, dopaminergic |
| Annotated paper: | Wang C, et al. "Synaptotagmin-11 is a critical mediator of parkin-linked neurotoxicity and Parkinson's disease-like pathology" Nat Commun. 2018 Jan 8;9(1):81 PMID:29311685 |
| Figure(s): | Fig.1, 7, 8 |
| Annotation description: | Fig.1: "Effect of Parkin dysfunction on Syt11 accumulation" - "Furthermore, the parkin-mediated decrease of Syt11 level was prevented by 12-h pretreatment with the proteasome inhibitor MG132 (Fig. 1d), confirming that parkin-mediated Syt11 degradation occurs through the proteasome pathway." [Fig.5: "Syt11 inhibits vesicle pool replenishment in dopaminergic neurons"] Fig.7: "Syt11 knockdown rescues parkin knockdown-induced deficits in DA release and motor behavior." Fig.8: "Expression of Syt11 in dopaminergic neurons is critical for parkin to mediate DA release and motor deficits." Note: Syt11 is a known regulator of dopamine SV endocytosis. |
| Evidence tracking, Biological System: | Intact tissue Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression |
| Evidence tracking, Experiment Assay: | Western blot IP + WB/MSMS Voltammetry assay |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 3784 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |