| Annotated protein: | Myc box-dependent-interacting protein 1 (Amphiphysin II) (Amphiphysin-like protein) (Bridging integrator 1) (SH3 domain-containing protein 9). Gene symbol: BIN1. Taxonomy: Mus musculus (Mouse). Uniprot ID: O08539 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ BIN1 |
| Ontology domain: | Biological Process |
| SynGO term: | synaptic vesicle endocytosis (GO:0048488) |
| Synapse type(s): | neocortex |
| Annotated paper: | Di Paolo G, et al. "Decreased synaptic vesicle recycling efficiency and cognitive deficits in amphiphysin 1 knockout mice" Neuron. 2002 Feb 28;33(5):789-804 PMID:11879655 |
| Figure(s): | fig 4, fig 5 |
| Annotation description: | The authors generate an amphiphysin I KO mouse. In fig3 they demonstrate that uptake of the endocytic tracer FM2-10 is reduced in synaptosomes prepared from KO mice when compared to wild-type mice. In fig 5 the authors use EM to demonstrate that loading of synaptic vesicles with a fluid phase tracer, HRP is decreased in cortical neurons prepared from amphiphysin KO mice. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Optical physiology |
| Annotator(s): | Peter McPherson (ORCID:0000-0001-7806-5662) |
| Lab: | Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, QC H3A 2B4, Canada |
| SynGO annotation ID: | 3742 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |