| Annotated protein: | Vacuolar protein sorting-associated protein 35 (Maternal-embryonic 3) (Vesicle protein sorting 35). Gene symbol: VPS35. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9EQH3 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ VPS35 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of synapse maturation (GO:0090128) |
| Synapse type(s): | hippocampus, glutamatergic cerebral cortex |
| Annotated paper: | Tian Y, et al. "VPS35-deficiency results in an impaired AMPA receptor trafficking and decreased dendritic spine maturation" Mol Brain. 2015 Oct 31;8(1):70 PMID:26521016 |
| Figure(s): | fig 1, 2, 3d,e, 6, s1b |
| Annotation description: | From Results section: "Golgi staining analysis showed that the spine densities of both apical and basal dendrites of CA1 pyramidal neurons were reduced in VPS35+/m hippocampus, compared with that of WT controls (Figs. 1a, b). Further examining mature and immature spines (revealed by spine morphology as illustrated in Fig. 1c) demonstrated an obvious reduction of mature spines in VPS35-deficient CA1 hippocampal neurons (Fig. 1a, b). No difference was observed in spine density or morphology in cortical layer 2 pyramidal neurons between VPS35+/+ and +/m brain (Fig. 1d, e). In contrast, the spine density reduction was detected in cortical layer 5 pyramidal neurons of VPS35+/m brain (Fig. 1f, g)." "VPS35+/m hippocampal neurons displayed reduced spine numbers per 10 μm, compared with that of WT control neurons (Fig. 2a, b). In particular, the mature spines such as mushroom spines were largely decreased (Fig. 2c). However, the number of filopodia-like spines, which are usually considered as immature spines, was increased in VPS35-deficient neurons (Fig. 2c). A similar spine deficit was also detected in VPS35+/m hippocampal neurons at DIV 18 by phalloidin staining, which labels F-actin (Figs. 2d, e). Moreover, VPS35+/m cortical neurons showed the similar spine defect (Additional file 1: Figure S1B)." "We thus further analyzed the number of synapses in the hippocampus by transmission electron microscopy (EM). The excitatory synapse was indeed decreased in VPS35+/m hippocampus, compared with that of WT controls (Fig. 3d, e)." "...the spine deficit (e.g., reduced mature spines) due to VPS35-deficiency was restored by expression of GFP-GluA2, but not GFP-GluA1 (Fig. 6a-c)." 7/6/2018 Pim - I have made a second annotation to term 'regulation of synapse maturation' |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal |
| Annotator(s): | Rita Reig-Viader (ORCID:0000-0002-6893-6177) Àlex Bayés (ORCID:0000-0002-5265-6306) |
| Lab: | Molecular Physiology of the Synapse Laboratory, Biomedical Research Institute Sant Pau, 08025 Barcelona, Spain and and Universitat Autnoma de Cerdanyola del Valls, Spain Barcelona, 08193 Bellaterra |
| SynGO annotation ID: | 3735 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |