| Annotated protein: | Interleukin-1 receptor accessory protein-like 1 (IL-1-RAPL-1) (IL-1RAPL-1) (IL1RAPL-1) (EC 3.2.2.6) (Oligophrenin-4) (Three immunoglobulin domain-containing IL-1 receptor-related 2) (TIGIRR-2) (X-linked interleukin-1 receptor accessory protein-like 1). Gene symbol: IL1RAPL1. Taxonomy: Homo sapiens (Human). Uniprot ID: Q9NZN1 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ IL1RAPL1 |
| Ontology domain: | Biological Process |
| SynGO term: | trans-synaptic signaling by trans-synaptic complex (GO:0099545) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Valnegri P, et al. "The X-linked intellectual disability protein IL1RAPL1 regulates excitatory synapse formation by binding PTPdelta and RhoGAP2" Hum Mol Genet. 2011 Dec 15;20(24):4797-809 PMID:21926414 |
| Figure(s): | Fig. 1e,f,g,h; Fig. 2 |
| Annotation description: | Fig. 1e,f,g,h; overexpression of IL1RAPL1 in hippocampal neurons promotes excitatory but not inhibitory synapses formation Literal: "Only the extracellular domain of IL1RAPL1 was required to increase the excitatory (VGLUT1-positive, Fig. 1E and G), but not the inhibitory (VGAT-positive, Fig. 1F and H)," Fig. 2; IL1RAPL1 promote functional excitatory synapse formation Literal: "Finally, in IL1RAPL1 over-expressing neurons, but not in neurons over-expressing IL1RAPL1ΔN, an increase in the number of pre-synaptic contacts was associated with an increase of the miniature excitatory post-synaptic current (mEPSC) frequency (Fig. 2C and D" "COS-7 cells transfected with GFP (negative control), neuroligin-2 (positive control), HA-IL1RAPL1 (either fulllength or lacking the extracellular domain, IL1RAPL1ΔN) or HA-IL1RAPL2 were co-cultured with hippocampal neurons at DIV 8. After 3 days of co-culture, we fixed the cells, and the pre-synapses were visualized using an antibody against synapsin I. COS-7 cells expressing wild-type (wt) IL1RAPL1, IL1RAPL2 and neuroligin-2, but not IL1RAPL1ΔN, induced pre-synaptic differentiation in contacting axons, showing significantly higher levels of synapsin I intensity compared with the COS-7 cells expressing GFP (Fig. 2F and H," "However, IL1RAPL1-expressing COS-7 cells were not able to recruit VGAT-positive pre-synaptic terminals (Fig. 2G)." 7/6/2017 Pim - The term "presynapse assembly" is allowed over "synapse assembly" because of the experiments in non-neuronal cels where expressed IL1RAPL1 is able to induce aggregation of presynaptic marker proteins. |
| Evidence tracking, Biological System: | Cultured neurons Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| Additional literature: | Similar results were reported in this paper @ PMID:21940441 |
| SynGO annotation ID: | 3732 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |