| Annotated protein: | Syntaxin-4. Gene symbol: STX4. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q08850 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ STX4 |
| Ontology domain: | Biological Process |
| SynGO term: | exocytic insertion of neurotransmitter receptor to postsynaptic membrane (GO:0098967) |
| Synapse type(s): | Schaffer collateral synapse (CA3->CA1) |
| Annotated paper: | Kennedy MJ, et al. "Syntaxin-4 defines a domain for activity-dependent exocytosis in dendritic spines" Cell. 2010 Apr 30;141(3):524-35 PMID:20434989 |
| Figure(s): | Figures 6 and 7 |
| Annotation description: | FIGURE 6: Stx4 knock down blocks TfR-SEP membrane insertion following stimulation with Bic/Gly suggesting Syntaxin-4 mediates activity-triggered exocytosis in dendrites/spines FIGURE 7: Stx4 deltaTM prevents LTP in Schaffer collaterals. LTP was induced by depolarizing the cell to 0 mV and delivering 200 stimuli at 2 Hz. Using standard intracellular solution, this induction protocol reliably triggered robust sustained potentiation of EPSC amplitudes to 172 ± 18% of baseline (Figure 7A). However, LTP was almost completely abolished (EPSC amplitudes 111 ± 19% of baseline) when purified Stx4DTM (2.5 mM) was included in the recording solution. 19/6/2018 Pim - Fig.3: "AMPA Receptors are Directly Inserted Into the Spine Plasma Membrane" - Fig.5: "Stx4 Marks Sites of Exocytosis at the Spine Membrane" |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp |
| Annotator(s): | Ruud Toonen (ORCID:0000-0002-9900-4233) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 3723 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |